Protein interaction discovery using parallel analysis of translated ORFs (PLATO).
basic_science · Level V
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- Record sourced from PubMed, PMID 23503679.
- Also identified by DOI 10.1038/nbt.2539 and PMC identifier 4110636.
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Abstract
Identifying physical interactions between proteins and other molecules is a critical aspect of biological analysis. Here we describe PLATO, an in vitro method for mapping such interactions by affinity enrichment of a library of full-length open reading frames displayed on ribosomes, followed by massively parallel analysis using DNA sequencing. We demonstrate the broad utility of the method for human proteins by identifying known and previously unidentified interacting partners of LYN kinase, patient autoantibodies, and the small-molecules gefitinib and dasatinib.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Open Reading Frames
- Protein Biosynthesis
- Protein Interaction Mapping
- Protein Interaction Maps