A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.

Shaner, Nathan C; Lambert, Gerard G; Chammas, Andrew; Ni, Yuhui; Cranfill, Paula J; Baird, Michelle A; Sell, Brittney R; Allen, John R et al. · Nat Methods · 2013

basic_science · Level V

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Abstract

We report a monomeric yellow-green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum. mNeonGreen is the brightest monomeric green or yellow fluorescent protein yet described to our knowledge, performs exceptionally well as a fusion tag for traditional imaging as well as stochastic single-molecule superresolution imaging and is an excellent fluorescence resonance energy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

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