Introduction of the beta isozyme of protein kinase C accelerates induced differentiation of murine erythroleukemia cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 2352925.
- Also identified by PMC identifier 54125.
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Abstract
Induction of differentiation in murine erythroleukemia cells (MELCs) involves a protein kinase C (PKC)-mediated step. Vincristine-resistant cells respond more rapidly to hybrid polar/apolar inducers than the parental cells. These vincristine-resistant MELCs contain elevated levels of the beta isozyme of PKC (PKC-beta). Exogenous homologous murine PKC-beta, incorporated into permeabilized MELCs, accelerates induced differentiation. Neither rat PKC-beta, nor mouse PKC-alpha, nor rat PKC-alpha, incorporated into permeabilized MELCs, is effective in altering the kinetics of induced differentiation. This provides direct evidence for a rate-limiting role for this PKC isozyme during N,N'-hexamethylenebisacetamide-mediated induced differentiation of a transformed cell.
Medical subject headings
- Isoenzymes
- Protein Kinase C
- Tumor Cells, Cultured