Highly sensitive fluorescent protein FRET detection using optofluidic lasers.
basic_science · Level V
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- Record sourced from PubMed, PMID 23545541.
- Also identified by DOI 10.1039/c3lc50207d.
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Abstract
We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.
Medical subject headings
- Fluorescence Resonance Energy Transfer
- Lasers
- Luminescent Proteins
- Microfluidics
- Optics and Photonics
- Protein Interaction Mapping