Molecular-level dengue fever diagnostic devices made out of paper.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23563693.
- Also identified by DOI 10.1039/c3lc50135c.
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Abstract
This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.
Medical subject headings
- Dengue
- Dengue Virus
- Molecular Diagnostic Techniques
- Nucleic Acid Amplification Techniques
- Paper