Protein chemical synthesis by serine and threonine ligation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23569249.
- Also identified by DOI 10.1073/pnas.1221012110 and PMC identifier 3637748.
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Abstract
An efficient method has been developed for the salicylaldehyde ester-mediated ligation of unprotected peptides at serine (Ser) or threonine (Thr) residues. The utility of this peptide ligation approach has been demonstrated through the convergent syntheses of two therapeutic peptides--ovine-corticoliberin and Forteo--and the human erythrocyte acylphosphatase protein (∼11 kDa). The requisite peptide salicylaldehyde ester precursor is prepared in an epimerization-free manner via Fmoc-solid-phase peptide synthesis.
Medical subject headings
- Acid Anhydride Hydrolases
- Aldehydes
- Corticotropin-Releasing Hormone
- Peptides
- Protein Engineering
- Teriparatide