A simple tool to improve pluripotent stem cell differentiation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23584186.
- Also identified by DOI 10.1038/nmeth.2442 and PMC identifier 3694177.
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Abstract
We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in several cell lines.
Medical subject headings
- Cell Differentiation
- Induced Pluripotent Stem Cells