Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23592706.
- Also identified by DOI 10.1002/adhm.201300049 and PMC identifier 4103153.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.
Medical subject headings
- Cell Differentiation
- Cell Separation
- Coculture Techniques
- Feeder Cells
- Induced Pluripotent Stem Cells
- Myocytes, Cardiac
- Peptide Hydrolases
- Water