Inhibition of BCR/ABL protein expression by miR-203 sensitizes for imatinib mesylate.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23613955.
- Also identified by DOI 10.1371/journal.pone.0061858 and PMC identifier 3627914.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Selective inhibition of BCR/ABL expression by RNA interference has been demonstrated as an effective strategy in CML treatment and a reversal to imatinib resistance. microRNAs (miRNAs) are small regulatory RNAs involved in post-transcriptional gene regulation. miR-203 is supposed to directly regulate ABL and BCR/ABL expression, however, the role of miR-203 in imatinib-resistant cells is not clear. Here, we report that overexpression of miR-203 in BaF3-BCR/ABL cells with T315I mutant inhibited cell growth and colony formation ability. Furthermore, miR-203 increased sensitivity to imatinib in BaF3-BCR/ABL(T315I) cells, thereby antagonizing the main mechanism of resistance to imatinib.
Medical subject headings
- Benzamides
- Fusion Proteins, bcr-abl
- MicroRNAs
- Piperazines
- Pyrimidines