Comprehensive macromolecular conformations mapped by quantitative SAXS analyses.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23624664.
- Also identified by DOI 10.1038/nmeth.2453 and PMC identifier 3728378.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Comprehensive perspectives of macromolecular conformations are required to connect structure to biology. Here we present a small angle X-ray scattering (SAXS) Structural Similarity Map (SSM) and Volatility of Ratio (<i>V<sub>R</sub></i>) metric providing comprehensive, quantitative and objective (superposition-independent) perspectives on solution state conformations. We validate <i>V<sub>R</sub></i> and SSM utility on human MutSβ, a key ABC ATPase and chemotherapeutic target, by revealing MutSβ DNA sculpting and identifying multiple conformational states for biological activity.
Medical subject headings
- Protein Conformation
- Scattering, Small Angle
- X-Ray Diffraction