Clostridium botulinum strain Af84 contains three neurotoxin gene clusters: bont/A2, bont/F4 and bont/F5.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23637798.
- Also identified by DOI 10.1371/journal.pone.0061205 and PMC identifier 3625220.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters.
Medical subject headings
- Botulinum Toxins
- Botulinum Toxins, Type A
- Clostridium botulinum
- Multigene Family
- Neurotoxins
- Sequence Analysis