A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23637962.
- Also identified by DOI 10.1371/journal.pone.0062054 and PMC identifier 3639282.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.
Medical subject headings
- Chromosomes, Artificial, Bacterial
- Gene Knock-In Techniques
- Genetic Engineering
- Genetic Vectors
- Recombination, Genetic