Live mammalian cell arrays.

Woodruff, Kristina; Fidalgo, Luis M; Gobaa, Samy; Lutolf, Matthias P; Maerkl, Sebastian J · Nat Methods · 2013

basic_science · Level V

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Abstract

High-content assays have the potential to drastically increase throughput in cell biology and drug discovery, but handling and culturing large libraries of cells such as primary tumor or cancer cell lines requires expensive, dedicated robotic equipment. We developed a simple yet powerful method that uses contact spotting to generate high-density nanowell arrays of live mammalian cells for the culture and interrogation of cell libraries.

Medical subject headings