Differential effects of phosphatase inhibitors on the calcium homeostasis and migration of HaCaT keratinocytes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23646108.
- Also identified by DOI 10.1371/journal.pone.0061507 and PMC identifier 3640006.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Changes in intracellular calcium concentration ([Ca²⁺]i) as well as in the phosphorylation state of proteins have been implicated in keratinocyte wound healing revealed in scratch assays. Scratching confluent HaCaT monolayers decreased the number of cells displaying repetitive Ca²⁺ oscillations as well as the frequency of their Ca²⁺-transients in cells close to the wounded area and initiated migration of the cells into the wound bed. In contrast, calyculin-A (CLA) and okadaic acid (OA), known cell permeable inhibitors of protein phosphatase-1 and 2A, increased the level of resting [Ca²⁺]i and suppressed cell migration and wound healing of HaCaT cells. Furthermore, neither CLA nor OA influenced how scratching affected Ca²⁺ oscillations. It is assumed that changes in and alterations of the phosphorylation level of Ca²⁺-transport and contractile proteins upon phosphatase inhibition mediates cell migration and wound healing.
Medical subject headings
- Calcium Signaling
- Enzyme Inhibitors
- Homeostasis
- Keratinocytes
- Phosphoprotein Phosphatases