Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23650528.
- Also identified by DOI 10.1371/journal.pone.0062771 and PMC identifier 3641123.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.
Medical subject headings
- Antineoplastic Agents
- Apoptosis
- Transcriptome
- Tretinoin