Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23657866.
- Also identified by DOI 10.1002/jbma.34695.
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Abstract
The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water-holding capability. These results suggest that the Dextran-coated PVF sponges with high water-holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial.
Medical subject headings
- Bone Marrow Cells
- Coated Materials, Biocompatible
- Dextrans
- Osteogenesis
- Stem Cells
- Water