Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
basic_science · Level V
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- Record sourced from PubMed, PMID 23661761.
- Also identified by DOI 10.1126/science.1233989.
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Abstract
Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.
Medical subject headings
- Antibodies, Neutralizing
- HIV Antibodies
- HIV Infections
- HIV-1