TALEN-mediated editing of the mouse Y chromosome.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23666012.
- Also identified by DOI 10.1038/nbt.2595 and PMC identifier 3681814.
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Abstract
The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
Medical subject headings
- Endonucleases
- Proteins
- SOXB2 Transcription Factors
- Y Chromosome