Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23667548.
- Also identified by DOI 10.1371/journal.pone.0062947 and PMC identifier 3647075.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present an integrated approach for efficient characterization of intrinsically disordered proteins. Batch cell-free expression, fast data acquisition, automated analysis, and statistical validation with data resampling have been combined for achieving cost-effective protein expression, and rapid automated backbone assignment. The new methodology is applied for characterization of five cytosolic domains from T- and B-cell receptors in solution.
Medical subject headings
- Intrinsically Disordered Proteins
- Nuclear Magnetic Resonance, Biomolecular
- Receptors, Antigen, B-Cell
- Receptors, Antigen, T-Cell