Mass spectrometry-based metabolomics of single yeast cells.
basic_science · Level V
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- Record sourced from PubMed, PMID 23671112.
- Also identified by DOI 10.1073/pnas.1209302110 and PMC identifier 3670324.
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Abstract
Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (ΔPFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
Medical subject headings
- Glycolysis
- Metabolomics
- Saccharomyces cerevisiae
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization