RESTseq--efficient benchtop population genomics with RESTriction Fragment SEQuencing.
Where this comes from
- Record sourced from PubMed, PMID 23691128.
- Also identified by DOI 10.1371/journal.pone.0063960 and PMC identifier 3656931.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We present RESTseq, an improved approach for a cost efficient, highly flexible and repeatable enrichment of DNA fragments from digested genomic DNA using Next Generation Sequencing platforms including small scale Personal Genome sequencers. Easy adjustments make it suitable for a wide range of studies requiring SNP detection or SNP genotyping from fine-scale linkage mapping to population genomics and population genetics also in non-model organisms. We demonstrate the validity of our approach by comparing two honeybee and several stingless bee samples.
Medical subject headings
- High-Throughput Nucleotide Sequencing
- Metagenomics
- Restriction Mapping