Functionalization of optical nanotip arrays with an electrochemical microcantilever for multiplexed DNA detection.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23695411.
- Also identified by DOI 10.1039/c3lc50335f.
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Abstract
Optical nanotip arrays fabricated on etched fiber bundles were functionalized with DNA spots. Such unconventional substrates (3D and non-planar) are difficult to pattern with standard microfabrication techniques but, using an electrochemical cantilever, up to 400 spots were electrodeposited on the nanostructured optical surface in 5 min. This approach allows each spot to be addressed individually and multiplexed fluorescence detection is demonstrated. Finally, remote fluorescence detection was performed by imaging through the optical fiber bundle itself after hybridisation with the complementary sequence.
Medical subject headings
- Biosensing Techniques
- DNA
- Electrochemistry
- Fiber Optic Technology
- Nucleic Acid Hybridization