Two-photon ratiometric fluorescent mapping of intracellular transport pathways of pH-responsive block copolymer micellar nanocarriers.

Liu, Tao; Hu, Jinming; Jin, Zhenyu; Jin, Fan; Liu, Shiyong · Adv Healthc Mater · 2013

basic_science · Level V

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Abstract

Ultrasensitive ratiometric fluorescent pH probes based on dual dye-labeled pH-responsive diblock copolymer micellar scaffold are constructed. The pH-sensitive emitting nature of BTPE dyes and emission turn-on of CMA moieties triggered by pH-actuated micelle-to-unimer of diblock scaffold synergistically contribute to the observed ≈250-fold changes of BTPE/CMA emission intensity ratios in the whole pH range. Two-photon ratiometric fluorescent pH mapping of intracellular gradients subjected by pH-responsive micellar nanoparticles in their endocytic pathway has been thus achieved.

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