Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23704970.
- Also identified by DOI 10.1371/journal.pone.0064017 and PMC identifier 3660326.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
Medical subject headings
- Azo Compounds
- Genes, Viral
- Orthomyxoviridae
- Peptide Nucleic Acids
- Viral Nonstructural Proteins