MicroRNA target site identification by integrating sequence and binding information.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23708386.
- Also identified by DOI 10.1038/nmeth.2489 and PMC identifier 3818907.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute proteins can pinpoint a microRNA (miRNA) target site within tens of bases but leaves the identity of the miRNA unresolved. A flexible computational framework, microMUMMIE, integrates sequence with cross-linking features and reliably identifies the miRNA family involved in each binding event. It considerably outperforms sequence-only approaches and quantifies the prevalence of noncanonical binding modes.
Medical subject headings
- Algorithms
- Protein Interaction Mapping
- RNA
- RNA-Binding Proteins
- Sequence Analysis, RNA