Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23708387.
- Also identified by DOI 10.1038/nmeth.2488 and PMC identifier 3696415.
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Abstract
Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras have the potential to dramatically accelerate data acquisition, enlarge the field of view and increase the effective quantum efficiency in single-molecule switching nanoscopy. However, sCMOS-intrinsic pixel-dependent readout noise substantially lowers the localization precision and introduces localization artifacts. We present algorithms that overcome these limitations and that provide unbiased, precise localization of single molecules at the theoretical limit. Using these in combination with a multi-emitter fitting algorithm, we demonstrate single-molecule localization super-resolution imaging at rates of up to 32 reconstructed images per second in fixed and living cells.
Medical subject headings
- Algorithms
- Image Enhancement
- Microscopy, Video
- Molecular Imaging
- Nanotechnology
- Pattern Recognition, Automated
- Semiconductors