Evaluation of LDH-A and glutaminase inhibition in vivo by hyperpolarized 13C-pyruvate magnetic resonance spectroscopy of tumors.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23722553.
- Also identified by DOI 10.1158/0008-5472.CAN-13-0465 and PMC identifier 3736580.
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Abstract
Hyperpolarized (13)C magnetic resonance spectroscopy provides a unique opportunity to detect real-time metabolic fluxes as a means to measure metabolic treatment responses in vivo. Here, we show that pharmacologic inhibition of lactate dehydrogenase-A suppressed the conversion of hyperpolarized (13)C-pyruvate to lactate in murine xenografts of P493 human lymphoma. In contrast, a glutaminase inhibitor reduced conversion of (13)C-pyruvate to alanine without affecting conversion of pyruvate to lactate. These results illustrate the ability to monitor biomarkers for responses to antimetabolic therapy in real-time, paving the way for clinical development of imaging biomarkers to monitor metabolic pharmacodynamics.
Medical subject headings
- Carbon Isotopes
- Glutaminase
- L-Lactate Dehydrogenase
- Lymphoma
- Magnetic Resonance Spectroscopy
- Pyruvic Acid