A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C.
basic_science · Level V
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- Record sourced from PubMed, PMID 23723239.
- Also identified by DOI 10.1126/science.1235532 and PMC identifier 3763809.
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Abstract
Chromosome segregation during mitosis requires assembly of the kinetochore complex at the centromere. Kinetochore assembly depends on specific recognition of the histone variant CENP-A in the centromeric nucleosome by centromere protein C (CENP-C). We have defined the determinants of this recognition mechanism and discovered that CENP-C binds a hydrophobic region in the CENP-A tail and docks onto the acidic patch of histone H2A and H2B. We further found that the more broadly conserved CENP-C motif uses the same mechanism for CENP-A nucleosome recognition. Our findings reveal a conserved mechanism for protein recruitment to centromeres and a histone recognition mode whereby a disordered peptide binds the histone tail through hydrophobic interactions facilitated by nucleosome docking.
Medical subject headings
- Centromere
- Chromosomal Proteins, Non-Histone
- Histones
- Nucleosomes