Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles.

Wang, Zongxing; Wu, Hung-jen; Fine, Daniel; Schmulen, Jeffrey; Hu, Ye; Godin, Biana; Zhang, John X J; Liu, Xuewu · Lab Chip · 2013

basic_science · Level V

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Abstract

We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.

Medical subject headings