Whole-rat conditional gene knockout via genome editing.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23749298.
- Also identified by DOI 10.1038/nmeth.2516.
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Abstract
Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.
Medical subject headings
- Deoxyribonucleases
- Gene Knockout Techniques
- Genome
- Rats
- Transfection
- Zinc Fingers