Integrated proteomic analysis of post-translational modifications by serial enrichment.
basic_science · Level V
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- Record sourced from PubMed, PMID 23749302.
- Also identified by DOI 10.1038/nmeth.2518 and PMC identifier 3943163.
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Abstract
We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
Medical subject headings
- Gene Expression Profiling
- Mass Spectrometry
- Protein Processing, Post-Translational
- Proteome