MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23762407.
- Also identified by DOI 10.1371/journal.pone.0065671 and PMC identifier 3675065.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
MiR-7 acts as a tumour suppressor in many cancers and abrogates proliferation of CHO cells in culture. In this study we demonstrate that miR-7 targets key regulators of the G1 to S phase transition, including Skp2 and Psme3, to promote increased levels of p27(KIP) and temporary growth arrest of CHO cells in the G1 phase. Simultaneously, the down-regulation of DNA repair-specific proteins via miR-7 including Rad54L, and pro-apoptotic regulators such as p53, combined with the up-regulation of anti-apoptotic factors like p-Akt, promoted cell survival while arrested in G1. Thus miR-7 can co-ordinate the levels of multiple genes and proteins to influence G1 to S phase transition and the apoptotic response in order to maintain cellular homeostasis. This work provides further mechanistic insight into the role of miR-7 as a regulator of cell growth in times of cellular stress.
Medical subject headings
- Adenosine Triphosphatases
- Autoantigens
- Cyclin-Dependent Kinase Inhibitor p27
- G1 Phase Cell Cycle Checkpoints
- MicroRNAs
- Proteasome Endopeptidase Complex
- S-Phase Kinase-Associated Proteins