Genome-wide profiling of human cap-independent translation-enhancing elements.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23770754.
- Also identified by DOI 10.1038/nmeth.2522 and PMC identifier 3731418.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.
Medical subject headings
- Genome, Human
- Peptide Chain Initiation, Translational
- RNA, Messenger