Near-infrared fluorescent proteins for multicolor in vivo imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23770755.
- Also identified by DOI 10.1038/nmeth.2521 and PMC identifier 3737237.
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Abstract
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs--iRFP670, iRFP682, iRFP702 and iRFP720--from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.
Medical subject headings
- Diagnostic Imaging
- Luminescent Proteins
- Microscopy, Fluorescence
- Phytochrome