Towards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 23776617.
- Also identified by DOI 10.1371/journal.pone.0066110 and PMC identifier 3680450.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure. Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA. Our results raise possibility of using rHSP70 for the serodiagnosis of TL.
Medical subject headings
- Leishmania
- Leishmaniasis
- Protozoan Proteins
- Recombinant Proteins
- Serologic Tests