Identification of pVHL as a novel substrate for Aurora-A in clear cell renal cell carcinoma (ccRCC).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23785518.
- Also identified by DOI 10.1371/journal.pone.0067071 and PMC identifier 3681765.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer and is often characterized by mutations or deletions of the Von Hippel Lindau (VHL) tumour suppressor gene. Aurora gene family members are implicated in proper mitotic progression and spindle checkpoint function and play a crucial role in cancer progression. In the present study, we assessed the expression of Aurora-A in a cohort of 30 ccRCC with fully characterized VHL status (wt/wt or mut/del) and Fuhrman grade. Aurora-A transcript and protein levels were significantly increased in high Fuhrman grade tumours and in VHLwt/wt tumours. These results suggest that Aurora-A and VHL interact in the ccRCC. We demonstrated that the two proteins interact in vivo and identified the Ser72 on the sequence of VHL as the unique site phosphorylated by Aurora-A.
Medical subject headings
- Aurora Kinase A
- Carcinoma, Renal Cell
- Kidney Neoplasms
- Von Hippel-Lindau Tumor Suppressor Protein