A reusable sensor for the label-free detection of specific oligonucleotides by surface plasmon fluorescence spectroscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23788367.
- Also identified by DOI 10.1002/adhm.201300056.
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Abstract
The development of a reusable molecular beacon (MB)-based sensor for the label-free detection of specific oligonucleotides using surface plasmon fluorescence spectroscopy (SPFS) as the readout method is described. The MBs are chemisorbed at planar gold surfaces serving as fluorescence quenching units. Target oligonucleotides of 24 bases can be detected within a few minutes at high single-mismatch discrimination rates.
Medical subject headings
- Oligonucleotides
- Surface Plasmon Resonance