Multiplexed MS/MS for improved data-independent acquisition.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23793237.
- Also identified by DOI 10.1038/nmeth.2528 and PMC identifier 3881977.
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Abstract
In mass spectrometry-based proteomics, data-independent acquisition (DIA) strategies can acquire a single data set useful for both identification and quantification of detectable peptides in a complex mixture. However, DIA data are noisy owing to a typical five- to tenfold reduction in precursor selectivity compared to data obtained with data-dependent acquisition or selected reaction monitoring. We demonstrate a multiplexing strategy, MSX, for DIA analysis that increases precursor selectivity fivefold.
Medical subject headings
- Peptides
- Proteomics
- Tandem Mass Spectrometry