Murine cell glycolipids customization by modular expression of glycosyltransferases.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23798992.
- Also identified by DOI 10.1371/journal.pone.0064728 and PMC identifier 3683021.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Functional analysis of glycolipids has been hampered by their complex nature and combinatorial expression in cells and tissues. We report an efficient and easy method to generate cells with specific glycolipids. In our proof of principle experiments we have demonstrated the customized expression of two relevant glycosphingolipids on murine fibroblasts, stage-specific embryonic antigen 3 (SSEA-3), a marker for stem cells, and Forssman glycolipid, a xenoantigen. Sets of genes encoding glycosyltansferases were transduced by viral infection followed by multi-color cell sorting based on coupled expression of fluorescent proteins.
Medical subject headings
- Antigens, CD
- Glycosyltransferases
- Lactosylceramides