The role of S100B in the interaction between adipocytes and macrophages.
basic_science · Level V
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- Record sourced from PubMed, PMID 23804363.
- Also identified by DOI 10.1002/oby.20532.
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Abstract
The S100 calcium binding protein B (S100B) implicated in brain inflammation acts via the receptor of advanced glycation end products (RAGE) and is also secreted from adipocytes. We investigated the role of S100B in the interaction between adipocytes and macrophages using a cell-culture model. RAW264.7 macrophages (RAW) were stimulated by recombinant S100B to observe alterations in TNF-α and M1 markers; 3T3-L1 adipocytes (L1) were stimulated by TNF-α to examine S100B secretion. RAW and L1 were then mutually stimulated with conditioned media of each other, or co-cultured. The effects of S100B silencing or a RAGE-neutralizing antibody were also investigated. S100B upregulated TNF-α and M1 markers in RAW, and TNF-α augmented S100B secretion from L1. L1 conditioned media stimulated TNF-α secretion from RAW, and RAW conditioned media increased S100B secretion from L1. The co-culture of RAW and L1 increased TNF-α, S100B, and the expression of M1 markers and the MCP-1 receptor CCR2. The silencing of S100B or RAGE neutralization significantly ameliorated TNF-α hypersecretion from RAW that were stimulated with L1 conditioned media. Thus, S100B as an adipokine may play a role in the interaction between adipocytes and macrophages to establish a vicious paracrine loop.
Medical subject headings
- 3T3-L1 Cells
- Adipocytes, White
- Adipocytes, White/drug effects
- Adipocytes, White/immunology
- Adipocytes, White/metabolism
- Adipokines
- Adipokines/antagonists & inhibitors
- Adipokines/genetics
- Adipokines/metabolism
- Animals
- Antibodies, Neutralizing
- Antibodies, Neutralizing/pharmacology
- Cell Communication
- Cell Communication/drug effects
- Cell Line, Transformed
- Coculture Techniques
- Culture Media, Conditioned
- Culture Media, Conditioned/metabolism
- Gene Silencing
- Immunomodulation
- Immunomodulation/drug effects
- Insulin Resistance
- Macrophage Activation
- Macrophage Activation/drug effects
- Macrophages
- Macrophages/drug effects
- Macrophages/immunology
- Macrophages/metabolism
- Mice
- Obesity
- Obesity/immunology
- Obesity/metabolism
- Paracrine Communication
- Paracrine Communication/drug effects
- Receptor for Advanced Glycation End Products
- Receptors, Immunologic
- Receptors, Immunologic/agonists
- Receptors, Immunologic/antagonists & inhibitors
- Receptors, Immunologic/metabolism
- Recombinant Proteins
- Recombinant Proteins/chemistry
- Recombinant Proteins/metabolism
- S100 Calcium Binding Protein beta Subunit
- S100 Calcium Binding Protein beta Subunit/antagonists & inhibitors
- S100 Calcium Binding Protein beta Subunit/genetics
- S100 Calcium Binding Protein beta Subunit/metabolism
- Signal Transduction
- Signal Transduction/drug effects
- Tumor Necrosis Factor-alpha
- Tumor Necrosis Factor-alpha/agonists
- Tumor Necrosis Factor-alpha/metabolism