Engineered nanostructured β-sheet peptides protect membrane proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23817067.
- Also identified by DOI 10.1038/nmeth.2533 and PMC identifier 3753066.
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Abstract
We designed β-strand peptides that stabilize integral membrane proteins (IMPs). β-strand peptides self-assemble in solution as filaments and become restructured upon association with IMPs; resulting IMP-β-strand peptide complexes resisted aggregation when diluted in detergent-free buffer and were visible as stable, single particles with low detergent background in electron micrographs. β-strand peptides enabled clear visualization of flexible conformations in the highly dynamic ATP-binding cassette (ABC) transporter MsbA.
Medical subject headings
- Membrane Proteins
- Nanostructures
- Peptides