Heritable genome editing in C. elegans via a CRISPR-Cas9 system.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23817069.
- Also identified by DOI 10.1038/nmeth.2532 and PMC identifier 3822328.
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Abstract
We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.
Medical subject headings
- Caenorhabditis elegans
- Genome
- Inverted Repeat Sequences
- RNA, Small Nuclear