A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA.
Where this comes from
- Record sourced from PubMed, PMID 23825578.
- Also identified by DOI 10.1371/journal.pone.0066864 and PMC identifier 3688954.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The study describes the method of a sensitive detection of double-stranded DNA molecules in situ. It is based on the oxidative attack on the deoxyribose moiety by copper(I) in the presence of oxygen. We have shown previously that the oxidative attack leads to the formation of frequent gaps in DNA. Here we have demonstrated that the gaps can be utilized as the origins for an efficient synthesis of complementary labeled strands by DNA polymerase I and that such enzymatic detection of the double-stranded DNA is a sensitive approach enabling in-situ detection of both the nuclear and mitochondrial genomes in formaldehyde-fixed human cells.
Medical subject headings
- Cell Nucleus
- DNA
- DNA, Mitochondrial