Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23826310.
- Also identified by DOI 10.1371/journal.pone.0067509 and PMC identifier 3694883.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.
Medical subject headings
- Anti-Bacterial Agents
- Genes, Bacterial
- Peptide Nucleic Acids
- RNA Interference
- RNA, Antisense
- Streptomyces coelicolor