A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.
basic_science · Level V
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- Record sourced from PubMed, PMID 23828485.
- Also identified by DOI 10.1039/c3lc50276g and PMC identifier 3755953.
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Abstract
We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Åvia on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.
Medical subject headings
- Alkaline Phosphatase
- Crystallography, X-Ray
- Microfluidic Analytical Techniques
- Temperature