Development and characterization of a chemically defined food for Drosophila.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23844001.
- Also identified by DOI 10.1371/journal.pone.0067308 and PMC identifier 3699577.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.
Medical subject headings
- Drosophila melanogaster
- Food, Formulated
- Larva
- Nutritional Requirements
- Reproduction
- Zygote