Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23861913.
- Also identified by DOI 10.1371/journal.pone.0068509 and PMC identifier 3701686.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust selection of transfected/transduced cells in the presence of Nourseothricin. NAT is compatible with other selection markers puromycin, hygromycin, neomycin, blasticidin, and is a valuable addition to the repertoire of mammalian selection markers.
Medical subject headings
- Acetyltransferases
- Anti-Bacterial Agents
- Streptothricins
- Transfection