Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol.
Where this comes from
- Record sourced from PubMed, PMID 23869230.
- Also identified by DOI 10.1371/journal.pone.0068739 and PMC identifier 3711900.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.
Medical subject headings
- Feces
- Microbiota
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA