Silicon dioxide thin film mediated single cell nucleic acid isolation.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23874571.
- Also identified by DOI 10.1371/journal.pone.0068280 and PMC identifier 3707904.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube.
Medical subject headings
- Membranes, Artificial
- Nucleic Acids
- Polymerase Chain Reaction
- Silicon Dioxide
- Single-Cell Analysis