Dynamic recruitment of CDK5RAP2 to centrosomes requires its association with dynein.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 23874654.
- Also identified by DOI 10.1371/journal.pone.0068523 and PMC identifier 3714271.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
CDK5RAP2 is a centrosomal protein known to be involved in the regulation of the γ-tubulin ring complex and thus the organization of microtubule arrays. However, the mechanism by which CDK5RAP2 is itself recruited to centrosomes is poorly understood. We report here that CDK5RAP2 displays highly dynamic attachment to centrosomes in a microtubule-dependent manner. CDK5RAP2 associates with the retrograde transporter dynein-dynactin and contains a sequence motif that binds to dynein light chain 8. Significantly, disruption of cellular dynein-dynactin function reduces the centrosomal level of CDK5RAP2. These results reveal a key role of the dynein-dynactin complex in the dynamic recruitment of CDK5RAP2 to centrosomes.
Medical subject headings
- Centrosome
- Dyneins
- Intracellular Signaling Peptides and Proteins
- Nerve Tissue Proteins